What is WST assay?
WST-1 Assay Reagent ab155902 provides a simple, accurate and ready-to-use assay to measure cell proliferation, cell viability and cytotoxicity in mammalian cells. The WST-1 assay protocol is based on the cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases.
What does WST-1 stand for?
Water Soluble Tetrazolium Salts
Water Soluble Tetrazolium Salts (WSTs) WST-1,-3,-4,-5,-8,-9.
What is MTT cell viability assay?
The MTT assay is used to measure cellular metabolic activity as an indicator of cell viability, proliferation and cytotoxicity. It is a quantitative assay that allows rapid and convenient handling of a high number of samples.
What is cell viability test?
Cell viability is a measure of the proportion of live, healthy cells within a population. Typically, cell viability assays provide a readout of cell health through measurement of metabolic activity, ATP content, or cell proliferation.
What is WST biology?
The Cell Proliferation Reagent WST-1 is designed to be used for the non-radioactive, spectrophotometric quantification of cell proliferation, growth, viability, and chemosensitivity in cell populations using the 96-well-plate format. The assessment of growth inhibitory antibodies and physiological mediators.
What is the full form of WST?
WST Full Form
| Full Form | Category | Term |
|---|---|---|
| WASHERMANPET | Indian Railway Station | WST |
| Weighted Settling Time | Chemistry | WST |
| (western) Samoan Time [from 2011-12-30; Utc +1300] | Time | WST |
| Western Brazil Standard Time [utc – 0400] | Time | WST |
Why are cell viability assays important?
The measurement of cell viability plays an essential role in all forms of cell culture. Sometimes, it is the sole purpose of the experiment, for example, in cytotoxicity assays. Alternatively, cell viability can be used to correlate cell behavior to cell number, providing a more accurate picture of cell metabolism.
What is the WST-1 cell viability assay?
WST-1 Cell Viability & Proliferation Assay utilizes a tetrazolim salt WST-1[2-(4-Iodophenyl)-3-(4-nitrophenyl)- 5-(2,4-disulfophenyl)-2H-tetrazolium]. WST-1 produces a highly water soluble formazan upon metabolically active cells, allowing a direct and user-friendly colorimetric measurement of cell viability and proliferation.
What is a synonym for viability?
Synonyms & Near Synonyms for viability. existence. survival, survivance, survivorship. enduringness, permanence. abidance, ceaselessness,
What is the colorimetric WST-1 assay?
The colorimetric WST-1 kit was purchased from Roche Diagnostics GmbH (Mannheim, Germany). In the WST-1 assay, the amount of formazan dye formed is directly related to the metabolic activity of cells. The assay was carried out as described in the manufacturer’s instructions.
What is a colorimetric assay for cell viability?
A colorimetric assay for quantifying cell viability and cell proliferation was carried out based on the cleavage of WST-1 into formazan by mitochondrial dehydrogenases in viable cells. LNCaP and HaCaT cells were seeded in growth medium at a density of 1 × 10 4 per well into a 96-well plate.