What mutation will occur as a result of DNA replication in the presence of 8-oxoG?
G → T transversion
Following a subsequent round of replication this misincorporation event results in a G → T transversion, which is the characteristic mutation caused by 8-oxoG.
What is the biological consequence if 8 Oxo G lesions are not removed from DNA?
If not repaired, 8-oxoG can pair with adenine and cause a G:C to T:A transversion. When 8-oxoG is inserted during DNA replication, it could generate double-strand breaks, which makes this lesion particularly deleterious.
What mutation will 8-Oxoguanine lead to?
8-Oxoguanine, a major oxidized base lesion formed by reactive oxygen species, causes G to T transversion mutations or leads to cell death in mammals if it accumulates in DNA.
How is 8-Oxoguanine formed?
8-oxoguanine (8-oxoG) is formed by the oxidation of a guanine base in DNA (Fig. 10.4). It is considered to be one of the major endogenous mutagens contributing broadly to spontaneous cell transformation. Its frequent miss-pairing with adenine during replication increases the number of G–C → T–A transversion mutations.
What base does 8-Oxoguanine pair with during DNA replication How might this cause mutation?
How does 8-Oxoguanine get into DNA?
In the mutagenesis pathway, 8-oxoguanine (GO) accumulates in DNA, via the incorporation of 8-oxo-dGTP from the nucleotide pool or because of direct oxidation of DNA. This increases the occurrence of A:T to C:G or G:C to T:A transversion mutations after two rounds of replication.
What is 8-Oxoguanine base pair?
8-Oxoguanine (8-hydroxyguanine, 8-oxo-Gua, or OH8Gua) is one of the most common DNA lesions resulting from reactive oxygen species modifying guanine, and can result in a mismatched pairing with adenine resulting in G to T and C to A substitutions in the genome.
How does 8 Oxoguanine get into DNA?
What is depurination in biology?
Depurination involves the loss of purine bases (adenine and guanine) from DNA. In spontaneously occurring depurination reactions, the N-glycosyl bound to deoxyribose is broken by hydrolysis, leaving the DNA’s sugar–phosphate chain intact, producing an abasic site.
What is depurination in biochemistry?
Depurination is a term usually applied to the loss of a purine (which is more common) or a pyrimidine, leading to an abasic site (also called an apurinic site for the same reason). Abasic sites are the most common modification in the genome in most organisms, including humans.