What is micF gene?
The micF gene is a stress response gene found in Escherichia coli and related bacteria that post-transcriptionally controls expression of the outer membrane porin gene ompF.
What is the main function of antisense RNA?
Antisense RNA molecule represents a unique type of DNA transcript that comprises 19–23 nucleotides and is complementary to mRNA. Antisense RNAs play the crucial role in regulating gene expression at multiple levels, such as at replication, transcription, and translation.
What is the meaning of antisense RNA?
Antisense RNA (asRNA), also referred to as antisense transcript, natural antisense transcript (NAT) or antisense oligonucleotide, is a single stranded RNA that is complementary to a protein coding messenger RNA (mRNA) with which it hybridizes, and thereby blocks its translation into protein.
How do you synthesize antisense RNA?
Antisense RNA probes are sequences of single-stranded RNA complementary to the coding sequence of the desired target mRNA. Antisense RNA is synthesized by in-vitro transcription using highly specific DNA-dependent RNA polymerases derived from bacteriophages such as SP6, T7, and T3 polymerases.
What does antisense RNA bind to?
Antisense RNAs directly bind to the coding region of sense RNA, resulting in direct inhibition of translation or mRNA destabilization.
Is mRNA an antisense?
Antisense is the non-coding DNA strand of a gene. A cell uses antisense DNA strand as a template for producing messenger RNA (mRNA) that directs the synthesis of a protein. Antisense can also refer to a method for silencing genes.
How does antisense RNA inhibit transcription?
Antisense transcripts and/or the act of antisense transcription regulate gene expression and genome integrity by interfering with sense transcription and modulating histone modifications or DNA methylation. Hence, they have significant pathological and physiological relevance.
How does antisense RNA regulate the expression of DNA?
Antisense RNAs are utilized for gene regulation and specifically target mRNA molecules that are used for protein synthesis. coli utilizing this system can regulate the expression of hok (toxin) and inhibits its translation by producing sok RNA (antitoxin). The outcome is the repression of hok mRNA translation.
How does antisense RNA inhibit translation?
Summary. Most antisense RNAs in bacteria inhibit translation by competing with ribosomes for translation initiation regions (TIRs) on nascent mRNA. This may involve ribosome sliding to a transiently open tisB TIR. IstR-1 competes with ribosomes by base pairing to the standby site located ∼100 nucleotides upstream.
Why is it called sense strand?
Gene Expression: Transcription of the Genetic Code It is also called sense strand, because the RNA sequence is the sequence that we use to determine what amino acids are produced through mRNA. As the RNA polymerase moves along the template strand in 3′→ 5′ direction, the RNA chain grows in 5′→ 3′ direction.
How can antisense RNA technology inhibit the expression of a gene?
Antisense RNA technology is one of the approaches that are used for the inhibition of gene expression or downregulation of a gene. This technology works on the principle that an antisense nucleic acid sequence base pairs with its complementary sense RNA strand and prevents it from being translated into protein.
What is the function of micF RNA?
MicF RNA. Predicted secondary structure and sequence conservation of MicF. The micF RNA is a non-coding RNA stress response gene found in Escherichia coli and related bacteria that post-transcriptionally controls expression of the outer membrane porin gene ompF.
Is MicF RNA a virulence factor in E coli?
Because of reduced OmpF levels, E. coli cells are more resistant to killing by NO within activated macrophages. Confirmation of this effect is needed and can come from analyses on killing in a micF mutant strain. It is thus possible that micF RNA is a virulence factor.
How did the micF promoter evolve to activate stress response genes?
The micF promoter may have evolved along with other promoters to respond to global transcriptional regulators which activate a spectrum of stress response genes.
What controls the expression of micF?
Expression of micF is controlled by both environmental and internal stress factors. Four transcriptional regulators are known to bind the micF promoter region and activate micF expression.