What is BD flow cytometry?
The BD FACSCelesta multi-colour cell analyzer helps rapidly and accurately analyze cells in a compact, affordable package. The BD FACSMelody cell sorter makes the complex world of flow cytometry and sorting surprisingly simple for more researchers, enabling deep scientific insights, lab efficiency and cost savings.
What is BD horizon?
BD Horizon™ Red 718 (R718), developed exclusively for BD Biosciences, is a small-molecule dye excited by the red (640 or 628 nm) laser with an emission maximum of 718 nm.
Does flow cytometry use fluorescent labeled antibodies?
In flow cytometry, cell subsets are labeled using a fluorescent antibody to a membrane protein. The fluorogen is activated by a laser as the cells pass by the detectors. Fluorescence in a flow cytometer is measured by a detector set at an angle to the light source.
What is flow cytometry technique?
Flow cytometry is a technology that rapidly analyzes single cells or particles as they flow past single or multiple lasers while suspended in a buffered salt-based solution. Each particle is analyzed for visible light scatter and one or multiple fluorescence parameters.
Can you use APC and PE together?
PerCP overlap a bit with APC and PeCy7. Pe-‐CF594 spill over a lot from and to Pe, don’t use together. PeCy5 spill over a lot to APC (bad), Pe and PeCy7. Avoid these combinations if possible.
Who owns BD Biosciences?
BD (company)
Trade name | BD |
---|---|
Founder | Maxwell Becton and Fairleigh S. Dickinson |
Headquarters | BD Headquarters Franklin Lakes, New Jersey , U.S. |
Area served | Worldwide |
Key people | Tom Polen (Chairman, CEO and President) Christopher R. Reidy (Executive Vice President and CAO) Christopher DelOrefice (Executive Vice President and CFO) |
Is BUV395 a tandem dye?
This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors. 355 nm 515/30 BD Horizon Brilliant™ Ultraviolet 496 (BUV496) (Exmax 348 nm/Emmax 496 nm) is a tandem fluorochrome that combines BD Horizon BUV395 and an acceptor dye with an Em Max at 496 nm.
Why are antibodies used in flow cytometry?
Since antibodies can recognize and bind to antigenic epitopes, they can be used to identify different molecules with specific epitopes. Also, high concentrations of antibodies in flow cytometry can lead to non-specific binding which will complicate the process.
How do you test fluorescent antibodies?
Immunofluorescence test By this technique, specific antibody is tagged with a fluorescent dye, allowed to react with the viral antigen in infected cells and after an incubation period, the antigen–antibody complex is visualized using a microscope with an ultraviolet light source.
How does direct fluorescent antibody work?
When labeled antibody is incubated with rabies-suspect brain tissue, it will bind to rabies antigen. Unbound antibody can be washed away and areas where antigen is present can be visualized as fluorescent-apple-green areas using a fluorescence microscope. If rabies virus is absent there will be no staining.