What is a TEF promoter?
Thyrotroph embryonic factor (TEF), a transcription factor, is a member of the PAR (proline and acidic amino acid-rich) subfamily of basic region/leucine zipper (bZIP) transcription factors. Indeed, TEF can bind to, and transactivate the TSHB promoter.
What is CYC1 Terminator?
CYC1 terminator is a DNA fragment with a special structure, a marker for end of transcription which is located in Saccharomyces cerevisiae strain S288c chromosome X from 526690bp to 526939bp . It is necessary for a vector to have this three parts, promoter, interest gene and terminator.
What is yeast promoter?
A promoter is a DNA sequence that can recruit transcriptional machinery and lead to transcription of the downstream DNA sequence. The specific sequence of the promoter determines the strength of the promoter (a strong promoter leads to a high rate of transcription initiation).
What is promoter analysis?
Promoter analysis can be done by analyzing the expression levels of RNA. The assumption is that genes that have similar expression levels have similar transcriptional regulation control and common binding sites. This knowledge is used we have to find promoter regions on genome sequences.
What is EM7 promoter?
EM7 promoter is just a small piece of random DNA when in your mammalian cell line. If your stable cells need blasticidin selection, they will depend upon the eukaryotic promoter (such as CMV, SV40), which is generally put in tandem with prokaryotic promoter (such as EM7, T7) for antibiotic expression in bacteria.
How do you stimulate a GAL promoter?
Just as traces of glucose repress the promoter, even small amounts of galactose will induce it. So you can try using 0.2% galactose in your medium, or if you want to be on the safe side, 1% of your sugar and 1% of galactose.
What is a Diauxic shift?
Description. The switch from rapid fermentative growth in the presence of a rich carbon source to slower exponential growth by aerobic respiration using ethanol once the preferred carbon source has been exhausted.
What is the GAL gene?
GAL (Galanin And GMAP Prepropeptide) is a Protein Coding gene. Diseases associated with GAL include Epilepsy, Familial Temporal Lobe, 8 and Acute Endometritis. Among its related pathways are Peptide ligand-binding receptors and Signaling by GPCR.
What happens when yeast undergoes a diauxic shift?
When yeast cells are grown in liquid cultures, they metabolize glucose predominantly by glycolysis, releasing ethanol in the medium. When glucose becomes limiting, the cells enter diauxic shift characterized by decreased growth rate and by switching metabolism from glycolysis to aerobic utilization of ethanol.
What is the promoter region of the TEF gene?
The promoter region of the A. gossypii TEF gene contains two tandemly arranged sequence elements that closely resemble the consensus sequence for the UASrpg from S. cerevisiae (Vignais et al. 1987, 1990), an enhancer motif found in the promoters of several highly expressed genes, among them the TEF genes.
What is the TEF promoter-iacz fusion of plasmid pag-111?
The TEF promoter-IacZ fusion of plasmid pAG-111 was derived from pPL1 by cleavage with HindIII, and therefore contains only 403 bp of the A. gossypii TEF promoter region. For construction of pAG-112 and pAG- 113, the TEF promoter-lacZ fusions were isolated from pPL2 and pPL3, respectively, after cutting with Xbal +SaiI.
Is a 9ossypii TEF gene a strong promoter?
The A. 9ossypii TEF gene was therefore expected to contain a strong promoter as has been shown for some of the TEF genes from other organism (Schirmaier and Philippsen 1984; Axelos et al. 1989; Kim et al. 1990; Ursin et al. 1991).
Is tef1 a Ste or Stea?
A conserved sequence in the TEF1 /2 transcript has been identified that also functions as a STE, suggesting that this sequence element maybe a general stability determinant found in other yeast mRNAs [8]. The cloned gene, called TEF1, encodes a protein of 458 amino acids (Mr = 50,071) in a single, uninterrupted reading frame [7].